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The 2018 Galaxy Community Conference (GCC2018) and Bioinformatics Open Source Conference 2018 (BOSC2018) are joining together for a unified event to be held in Portland, Oregon, United States, June 25-30, 2018.  The event features two days of training, a two day meeting, and finish with two or four days of intense collaboration at the GCC OBF CollaborationFest Core and Encore events.  The meeting will feature joint and parallel sessions, and shared keynotes, poster and demo sessions, and social events.  Colocating GCC and BOSC will bring together the widest possible community of bioinformatics developers and practitioners into a single place.  At this event the whole will be greater than the sum of the parts.

The event is organized by Oregon Health & Science University and will be held at Reed College, a small liberal arts college in Portland. Conference housing is available in two Reed College residence hall complexesPortland is a vibrant city in the American Northwest, close to the Cascade Mountains, the Oregon Coast, and the Columbia River. 

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Tuesday, June 26 • 12:30pm - 3:00pm
Setting up for success: Everything you need to know when planning for an RNA-seq analysis I

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Note: This is the first session of a two-session workshop.

This workshop is geared towards researchers who are thinking about conducting an RNA-seq experiment and are interested in knowing more about what is involved. The planning process requires taking a step back to evaluate various factors and ultimately assess the feasibility of the experiment, including thinking about potential pitfalls and how to avoid them. The workshop will go into detail about the different strategies for working with RNA-seq data depending on the biological question being addressed. Specific topics include:

  • Best practice guidelines for experimental design (Biological replicates, Paired-end vs Single-end, Sequencing depth).
  • Data storage and computational requirements.
  • Overview of commonly used workflows for differential gene expression, de-novo assembly, isoform quantification and other uses of RNA sequencing.

The focus of this workshop is to outline current standards and required resources for the analysis of RNA sequencing data. This workshop will not provide an exhaustive list of software tools or pipelines available; rather it aims to provide a fruitful discussion on how best to prepare for performing RNA-seq data analysis from the lab to manuscript preparation.


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Radhika S. Khetani

Harvard T.H. Chan School of Public Health

Meeta Mistry

Harvard T.H. Chan School of Public Health

Mary Piper

Harvard T.H. Chan School of Public Health

Tuesday June 26, 2018 12:30pm - 3:00pm
Training Venue 3