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Note: This is the first session of a two-session workshop.This
two-part workshop is geared towards researchers who are thinking about conducting an RNA-seq experiment and are interested in knowing more about what is involved. The planning process requires taking a step back to evaluate various factors and ultimately assess the feasibility of the experiment, including thinking about potential pitfalls and how to avoid them. The workshop will go into detail about the different strategies for working with RNA-seq data depending on the biological question being addressed. Specific topics include:
- Best practice guidelines for experimental design (Biological replicates, Paired-end vs Single-end, Sequencing depth).
- Data storage and computational requirements.
- Overview of commonly used workflows for differential gene expression, de-novo assembly, isoform quantification and other uses of RNA sequencing.
The focus of this workshop is to outline current standards and required resources for the analysis of RNA sequencing data. This workshop will not provide an exhaustive list of software tools or pipelines available; rather it aims to provide a fruitful discussion on how best to prepare for performing RNA-seq data analysis from the lab to manuscript preparation.
Prerequisites:
