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The 2018 Galaxy Community Conference (GCC2018) and Bioinformatics Open Source Conference 2018 (BOSC2018) are meeting together in Portland, Oregon, United States, June 25-30, 2018.  There are two days of training, a two day meeting, and two to four days of intense collaboration.  The meeting will feature joint & parallel sessions, shared keynotes, poster & demo sessions, birds-of-a-feather, and social events.  GCCBOSC brings together the widest possible community of bioinformatics developers and practitioners into a single event.  GCCBOSC is organized by Oregon Health & Science University and will be at Reed College.

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Tuesday, June 26 • 3:30pm - 6:00pm
Setting up for success: Everything you need to know when planning for an RNA-seq analysis II

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Note: This is the second session of a two-session workshop.


This two-part workshop is geared towards researchers who are thinking about conducting an RNA-seq experiment and are interested in knowing more about what is involved. The planning process requires taking a step back to evaluate various factors and ultimately assess the feasibility of the experiment, including thinking about potential pitfalls and how to avoid them. The workshop will go into detail about the different strategies for working with RNA-seq data depending on the biological question being addressed. Specific topics include:
  • Best practice guidelines for experimental design (Biological replicates, Paired-end vs Single-end, Sequencing depth).
  • Data storage and computational requirements.
  • Overview of commonly used workflows for differential gene expression, de-novo assembly, isoform quantification and other uses of RNA sequencing.
The focus of this workshop is to outline current standards and required resources for the analysis of RNA sequencing data. This workshop will not provide an exhaustive list of software tools or pipelines available; rather it aims to provide a fruitful discussion on how best to prepare for performing RNA-seq data analysis from the lab to manuscript preparation.
Prerequisites:
  • None

Speakers
RS

Radhika S. Khetani

Harvard T.H. Chan School of Public Health
MM

Meeta Mistry

Bioinformatics Analyst and Trainer, Harvard T.H Chan School of Public Health
I'm a wet-lab bench scientist turned bioinformatician who loves to play with data. When I am not consulting in bioinformatics analysis, I am teaching it.
MP

Mary Piper

Harvard T.H. Chan School of Public Health


Tuesday June 26, 2018 3:30pm - 6:00pm
Training Venue 3