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GCC BOSC 2018 has ended
The 2018 Galaxy Community Conference (GCC2018) and Bioinformatics Open Source Conference 2018 (BOSC2018) are meeting together in Portland, Oregon, United States, June 25-30, 2018.  There will be two days of training, a two+ day meeting, and four days of intense collaboration.  The meeting features joint & parallel sessions, shared keynotes, poster & demo sessions, birds-of-a-feather, and social events.  GCCBOSC is organized by Oregon Health & Science University and will be at Reed College.

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Thursday, June 28 • 4:01pm - 5:00pm
Closing Keynote: Confound it! Reproducible biology from "omics" data analysis

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Abstract:
High throughput sequencing (HTS) has become a preferred choice for the measurement of genome-wide biological phenomena at the molecular level, from genetics to gene expression regulation. Despite its widespread use, challenges remain in HTS data analysis. One often-overlooked aspect is normalization. Despite the fact that a variety of factors or “confounders” can contribute unwanted variation to the data, commonly used normalization methods often only correct for sequencing depth. The study of gene expression and gene expression regulation is particularly problematic when it is influenced simultaneously by a variety of biological factors in addition to the one of interest. Using examples from our lab and others, we show that confounders can dominate the signal of interest.  If the effect of confounders is not properly accounted for the power, reproducibility and biological insight of the results is compromised. I will focus on two methods aimed at correcting for confounding factors: RUVSeq (for RNA-seq) and DEScan (for epigenomic-seq). Our results show that removing confounding factors can make a dramatic difference on the biological conclusions of genome-wide studies of gene expression and epigenomic regulation.


Dr. Peixoto is an Assistant Professor at the Elson S. Floyd College of Medicine at Washington State University. Her research focuses on using genomic and computational biology approaches to study brain function. She also advises the WSU Spokane Genomics Core, and has published work on how analysis of complex datasets affects power and reproducibly in RNA-seq and Epigenomic-seq.

Lucia's keynote is sponsored by GCC.

Speakers
avatar for Lucia Peixoto

Lucia Peixoto

Assistant Professor, Elson S. Floyd College of Medicine, Washington State University
Dr. Peixoto is an Assistant Professor at the Elson S. Floyd College of Medicine at Washington State University. Her research focuses on using genomic and computational biology approaches to study brain function. In particular, understanding the underlying molecular basis of Autism Spectrum Disorders and their comorbidity with Intellectual Disability and Sleep Impairments. Her lab uses mouse... Read More →



Thursday June 28, 2018 4:01pm - 5:00pm
Vollum Lecture Hall Vollum College Center, Reed Campus

Attendees (42)